TY - JOUR AU - Buyun, Lyudmyla AU - Tkachenko, Halyna AU - Kurhaluk, Natalia AU - Gyrenko, Oleksandr AU - Kovalska, Lyudmyla AU - Góralczyk, Anna PY - 2021/05/31 Y2 - 2024/03/29 TI - Evaluation of Antibacterial Activity of the Ethanolic Extracts Derived from Leaves of Coelogyne brachyptera Rchb. f. (Orchidaceae) JF - Agrobiodiversity for Improving Nutrition, Health and Life Quality JA - – 1 –Agrobiodivers Improv Nutr Health Life Qua VL - 5 IS - 1 SE - Articles DO - UR - https://agrobiodiversity.uniag.sk/scientificpapers/article/view/335 SP - AB - <p style="text-align: justify;">The present study was aimed to assess the antibacterial activity of ethanolic extract obtained from leaves of<em> Coelogyne</em> <em>brachyptera</em> Rchb. f. For the current study, а panel of organisms including <em>Staphylococcus aureus</em> subsp. <em>aureus</em> Rosenbach (ATCC® 25923™) (mecA negative), <em>S. aureus</em> subsp. <em>aureus</em> Rosenbach (ATCC® 29213™) (mecA negative, Oxacillin sensitive, weak β-lactamase producing strain), <em>S. aureus</em> NCTC 12493 (mecA positive, Methicillin-resistant, EUCAST QC strain for cefoxitin), <em>Escherichia coli</em> (Migula) Castellani and Chalmers (ATCC® 25922™), <em>E. coli</em> (Migula) Castellani and Chalmers (ATCC® 35218™), <em>Pseudomonas aeruginosa</em> (Schroeter) Migula (ATCC® 27583™) were used. The antimicrobial susceptibility testing was done on Muller-Hinton agar by the disc diffusion method. The results of our study revealed the differential efficacy of ethanolic extract obtained from leaves of <em>C. brachyptera</em> on the test organisms. The ethanolic extract obtained from leaves of <em>C. brachyptera</em> revealed significant antibacterial activity against studied strains compared to control samples (96 % ethanol). A statistically significant increase (p &lt;0.05) in the inhibition zone diameters of strain growth was 47 % for <em>S. aureus</em> subsp. <em>aureus</em> ATCC® 25923™, 40 % for <em>S. aureus</em> subsp. <em>aureus</em> ATCC® 29213™, and 44 % for <em>S. aureus</em> NCTC 12493. A non-significantly increase in inhibition zone diameters of <em>E. coli</em> strains’ growth was also observed. The plant can be a source material to the herbal drug industry since it has some important antimicrobial components in the extracts that can be used for the development of therapeutic phytomedicine and phytoveterinary.</p> ER -