TY - JOUR AU - Petrovičová, Lenka AU - Balážová, Želmíra AU - Vivodík, Martin AU - Gálová, Zdenka PY - 2017/11/24 Y2 - 2024/03/29 TI - Separation of Rye Secalins by A-Page JF - Agrobiodiversity for Improving Nutrition, Health and Life Quality JA - – 1 –Agrobiodivers Improv Nutr Health Life Qua VL - IS - 1 SE - Articles DO - UR - https://agrobiodiversity.uniag.sk/scientificpapers/article/view/106 SP - AB - <p align="justify">The aim of our study was to evaluate the electrophoretic profiles of secalins of fifteen genotypes of rye (<em>Secale cereale </em>L.), which were obtained by polyacrylamide gel electrophoresis in the presence at pH 3.1 (A-PAGE). Electrophoretic separation of storage proteins was conducted according to the methodology recommended by an international organization ISTA, with some own modifications. Fractions from the preparative separation were pooled in such a way that no components from one pool were present in the others. Doc-It LS software was used to detect and to calculate variability of genotypes within individual rye species.Preparative electrophoresis at low pH allowed a simple separation of γ75k-secalins, ω-secalins and γ40k-secalins from the crude material under non-denaturing conditions. The content of γ75k-secalins varied in analyzed collection of rye from 22.86% (variety Čerkascanka tetra) to 53.93% (genotype Valticke) with an average 39.27%. The proportion of ω-secalins in our samples was in an average 39.27%. The largest percent representation of ω-secalins was proved in variety Česke (58.68%) and the lowest part of this subunits was detected in variety Radomske (25.83%). Our results showed that average representation of γ40k-secalins was 22.05%, with the highest content in variety Vigľašske (32.44%) overleaf with the lowest part of this fraction was detected in Valticke (8.28%). The variety of wheat Chinese spring and Marquis were used as standards. Storage proteins consist of three fractions, which are the main part of grain proteins and are used as a marker not only for genetic variability investigation, but also for characterization of genotypes. These pooled fractions could be used as starting material for single polypeptide purification.</p> ER -