Assessment of Oxidative Stress Biomarkers in the Equine Blood After In Vitro Incubation with Leaf Extract Obtained from Dendrobium Parishii Rchb.F.

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Lyudmyla Buyun
Halyna Tkachenko
Natalia Kurhaluk
Oleksandr Gyrenko
Lyudmyla Kovalska
Zbigniew Osadowski

Abstract

The main aim of the study was an assessment of the oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives content of protein oxidative modification, total antioxidant capacity] in the plasma and equine erythrocytes after treatment with Dendrobium parishii Rchb. f. leaf extract. The leaves of D. parishii plants, cultivated under glasshouse conditions, were sampled at M.M. Gryshko National Botanic Garden (NBG), National Academy of Science of Ukraine. Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in proportion 1:19, w/w). The equine plasma and erythrocyte aliquots were used in the study. The pellet of blood was resuspended in phosphate buffer (pH 7.4). A volume of 0.1 ml of the D. parishii extract was added to 1.9 ml of clean equine erythrocytes or 1.9 mL of plasma. For positive control (blank), phosphate buffer was used. After incubation of the mixture at 37°C for 60 min with continuous stirring, samples were used for the biochemical assays. The TBARS content as a biomarker of lipid peroxidation, aldehydic and ketonic derivatives level, as well as total antioxidant capacity, was non-significantly altered in the erythrocytes' suspension after in vitro incubation with an extract obtained from D. parishii. More significant changes were observed in the plasma. The D. parishii extract caused to increase in the formation of intracellular aldehydic and ketonic derivatives of oxidatively modified proteins in the extract-treated plasma, but these results were non-significant. Total antioxidant capacity was non-significant decreased both in plasma and erythrocytes. Screening of Dendrobium plants for other biological activities including antioxidant activities is essential and may be effective for searching the preventive agents in the pathogenesis of some metabolic diseases.

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How to Cite
Buyun, L., Tkachenko, H., Kurhaluk, N., Gyrenko, O., Kovalska, L., & Osadowski, Z. (2019). Assessment of Oxidative Stress Biomarkers in the Equine Blood After In Vitro Incubation with Leaf Extract Obtained from Dendrobium Parishii Rchb.F. Agrobiodiversity for Improving Nutrition, Health and Life Quality, (3). Retrieved from http://agrobiodiversity.uniag.sk/scientificpapers/article/view/300
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