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This study was aimed to investigate the biochemical composition of plant raw material of Artemisia spp. in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine at both budding and flowering stages. It was investigated the following species of Artemisia L. genus: A. abrotanum L., A. annua L., A. argyi H. Lev. & Vaniot, A. austriaca Jacq., A. japonica Thunb., A. ludoviciana Nutt., and A. maritima L. The content of dry matter determined according to A.I. Yermakov et al. (1972), the total content of reducing sugars, tannins, titratable acidity, and ascorbic acid according to V.P. Krishchenko (1983), the content of carotene according to B.P. Pleshkov (1985). At the stage of budding content of dry matter was from 26.72 (A. annua) to 48.63 (A. maritima) %, the content of reducing sugars from 5.11 (A. austriaca) to 8.93 (A. maritima) %, the titratable acidity from 2.06 (A. abrotanum) to 3.52 (A. japonica), the tannin content from 2.77 (A. abrotanum) to 5.1 (A. ludoviciana) %, ascorbic acid content from 11.65 (A. argyi) to 37.68 (A. japonica) mg%, and the content of carotene from 0.09 (A. ludoviciana) to 0.56 (A. abrotanum) mg%. At the stage of flowering, dry matter in raw was from 31.64 (A. annua) to 42.74 (A. austriaca) %, the content of sugars from 6.8 (A. austriaca) to 8.23 (A. annua) %, titratable acidity from 2.8 (A. abrotanum) to 4.66 (A. annua) %, tannin content from 4.22 (A. austriaca) to 6.36 (A. annua) %, the ascorbic acid content from 12.93 (A. abrotanum) to 65.18 (A. annua) mg%, and carotene content from 0.14 (A. austriaca) to 0.22 (A. annua) mg%.
Also, at the period of budding very strong correlation was between titratable acidity and tannin content (0.824), moderate correlation between dry matter and sugars content (0.581). At the stage of flowering determined a very strong correlation between sugars and tannin content (0.890), titratable acidity and tannins (0.957), titratable acidity and ascorbic acid content (0.999), tannins and ascorbic acid content (0.966).
In the M.M. Gryshko National Botanical Garden of the NAS of Ukraine just plants of A. abrotanum, A. annua, and A. austriaca passed in the period of flowering. Obtained data can be used for the deep further biochemical and pharmacological study.