Lipid Peroxidation and Total Antioxidant Capacity in the Muscle Tissue of Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus Mitchill) after In Vitro Treatment by Extracts Derived from Various Species of Dracaena Genus (Asparagaceae Juss.)

Abstract

Water extracts from selected Dracaena plants cultivated in greenhouse conditions were evaluated for antioxidant properties by in vitro methods using the muscle tissue of Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus Mitchill). The level of 2-thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) in the muscle tissue of Atlantic sturgeon after treatment in vitro by extracts derived from leaves of selected Dracaena plants (in final concentration 10 mg.mL^-1) were assessed. When muscle tissue was incubated with leaf extracts of various species belonging to the Dracaena genus, the TBARS level was significantly increased for the sixteen extracts studied. Moreover, all extracts (except D. singularis extract) increase the formation of TBARS in the extracts-treated muscle tissue, and these results were statistically significant. Treatment of muscle tissue by extracts derived from various species from the Dracaena genus revealed also increase the TAC level. When homogenates were incubated with leaf extracts derived from various species from the Dracaena genus, the TAC level was significantly increased for the fifteen extracts studied. Moreover, all extracts (except D. hyacinthoides and D. roxburghiana extracts) induced the TAC increase in the extracts-treated muscle tissue of Atlantic sturgeon, and these increases were statistically significant. It can be supposed that secondary plant metabolites, i.e. polyphenolic compounds, alkaloids, flavonoids, saponins, glycosides, terpenoids, tannins, etc., in extracts derived from the leaves of various species belonging to the Dracaena genus, may contribute to their antioxidant activity. Further detailed studies on the effect of extracts derived from leaves of selected Dracaena plants on long time intervals, antioxidant, and molecular aspects are necessary to understand the mechanism of action of extracts in other fish and animals.