Facets of the Elaboration of the Salvia sclarea L. Extracts

Abstract

In this article we presented some facets of the development of liquid ethanolic extracts of Salvia sclarea L. The primary purpose was to elaborate the analytical procedure of the quantitative determination of the total flavonoid content with the establishing dominating group of flavonoids in the extracts of Salvia sclarea by colorimetric aluminum chloride method. The second purpose of the work was to study the influence of pharmaceutical factors like temperature of extraction, particle size of the herbal raw material and ultrasound on the extraction of flavonoids. It was studied that the higher temperatures and ultrasound induced higher extraction of flavonoids from the herb of Salvia sclarea. The TFC was in the range of 6.4 to 14.7 mg rutin-equivalents per one gram of the herb and depended on temperature of extraction, particle size of herbal raw material and ultrasound on the extraction of flavonoids. Rutin was chosen as a commercially available marker. The calibration curve was plotted in the concentration range of 10 to 102 mg.L^-1 of rutin. The wavelength of maximum absorption of four extracts was in the range of 390–391 nm that indicated that flavons were dominating group of flavonoids in these extracts. These studies can be basis for the development of extracts of Salvia sclarea for pharmaceutical and cosmetic industries.