Biomarkers of Oxidative Stress in the Equine Erythrocytes after in vitro Treatment with Extracts Derived from Leaves and Pseudobulbs of Coelogyne pandurata Lindl. (Orchidaceae) Plants

Abstract

The current study was conducted to investigate the antioxidant properties of extracts derived from leaves and pseudobulbs of Coelogyne pandurata Lindl. using biomarkers of oxidative stress (2-thiobarbituric acid reactive substances (TBARS) as biomarkers of lipid peroxidation, aldehydic and ketonic derivatives of oxidative modification of proteins (OMP), total antioxidant capacity TAC) in the equine erythrocytes after in vitro treatment with the extracts. The obtained results demonstrated the prooxidative activity of C. pandurata extract used in the studied dose (5 mg.mL^-1) on the equine erythrocytes. Our results also showed that extract derived from the leaves and pseudobulbs of C. pandurata after incubation with erythrocyte samples caused to remaining the TAC level at a high level as compared to the group treated by phosphate buffer (controls), while levels of aldehydic and ketonic derivatives of OMP were unchanged. Our results also revealed that extracts derived from the leaves and pseudobulbs of C. pandurata after incubation with equine erythrocyte samples caused to increase in the TBARS level compared to untreated samples. Future studies will be conducted to evaluate dose-dependent changes in the levels of oxidative stress biomarkers after incubation with extracts derived from C. pandurata using various cell models. Moreover, the plant compound profile characteristics and antioxidant activity of different Coelogyne plants may encourage the wider use of these orchids in the development of new medicinal substances in medicine and veterinary.