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Abstract
Research on the resistance of cultivars of tobacco plants Nicotiana tabacum L. and Nicotiana rustica L. under conditions of artificial infection with necrotic and common isolates Potato virus Y (PVY). Molecular biological characteristics of Potato virus Y isolates were established by means of transmission electron microscopy, multiplex reverse polymerase chain reaction and ELISA. Biological characteristics of isolates were investigated by indicator plant’s method. The plant resistance of cultivars of tobacco to PVY isolates was investigated by artificial inoculation followed by visual detection of symptoms of infection and virus detection by transmission electron microscopy and ELISA. Studies of extracted from potato plants isolates showed that they belong to different strain groups in response to their infection, which developed in the indicator plant Nicotiana bentamiana Domin and Nicotiana tabacum var. Samsun. Multiplex RT-PCR confirmed availability of isolate Potato virus Y and the absence of other viruses that infect potatoes. Discovered different reactions tobacco plants var. Samsun 155 and Sobalchskyy 34/40 on inoculation of common and necrotic isolates of Potato virus Y. Plants of these cultivars respond at inoculation of necrotic isolate formation of systemic necrosis that were similar to the symptoms that caused this isolate to indicator plants. At inoculation of common isolate visible symptoms in plants of these cultivars were not observed, but the methods of transmission electron microscopy and ELISA confirmed the accumulation of the inoculated plants virions of Potato virus Y. However, there were no visible symptoms on inoculation with both isolates of plants Nacotiana rustica cultivars Matsui Field and Actec, and plant Nicotiana tabacum, variety Sobalchskyy 193. The plants cultivars Samsun 155 and Sobalchskyy 34/40 showed reaction tolerance relatively common isolate because the virus accumulates in them in large quantities in the absence of visual symptoms. However, these cultivars are susceptible to infection of the necrotic isolate of PVY. The absence of symptoms in plants Nacotiana rustica and tobacco cultivar Sobalchskyy 193 may indicate as tolerant reaction, and the presence of resistance to both isolates. Given the high harmfulness Potato virus Y and its wide spread in Ukraine, further studies are needed to identify the type of tobacco plant resistance that will make it possible to identify new sources of resistance to this pathogen.