Detection Genetic Variability of Castor Genotypes (Ricinus communis L.) Using SSR Markers

Abstract

Knowledge of genetic variability is important for breeding programs to provide the basis for developing desirable genotypes. The aim of this study was to assess genetic diversity within the set of 60 ricin genotypes using 5 SSR primers. Five SSR primers revealed a total of 38 alleles ranging from 5 to 10 alleles per locus with a mean value of 7.60 alleles per locus. The PIC values ranged from 0.776 (Rco40) to 0.872 (Rco33) with an average value of 0.843 and the DI value ranged from 0.786 (Rco40) to 0.876 (Rco33) with an average value of 0.849. The dendrogram of genetic similarity was constructed, based on UPGMA algorithm. The hierarchical cluster analysis divided castor genotypes into five main clusters. In this experiment, SSRs markers proved to be a high informative and usefull tool in genetic diversity research for the distinguishing and characterization of close related varieties.