In Vitro Antibacterial Efficacy of Different Natural Linden Honeys against Some Gram-Positive and Gram-Negative Strains

Abstract

Linden honey is rich in organic acids, such as formic acid and malic acid, which have antibacterial and antiinflammatory properties. This, in turn, helps prevent bacterial infections and inflammatory diseases that can lead to serious cardiovascular disorders. These bactericidal and antiviral properties are due to the high content of inhibin, lysozyme, and apidicin. These are bactericidal and antiviral enzymes that are responsible for the strong antibiotic properties of linden honey. The aim of the current study was in vitro antimicrobial profiling of different natural linden honey produced by Polish manufacturers, exhibiting inhibitory activity against Gram-positive strains such as Staphylococcus aureus subsp. aureus Rosenbach ATCC®25923™, and Gram-negative strains such as Pseudomonas aeruginosa (Schroeter) Migula ATCC®27853™, Escherichia coli (Migula) Castellani and Chalmers ATCC®25922™. The different natural linden honey produced by Polish manufacturers such as Beekeeping farm “Pszczółka“ (Ustka, Poland), Beekeeping farm “Mazurskie Miody Bogdan Piasecki“ (Tomaszkowo, Poland), Beekeeping farm “Sądecki Bartnik“ (Stróże, Poland), Beekeeping farm “Zaczarowany Ogród“, and Beekeeping farm “Karolczak Cezary” (Sławno, Poland) were used in the current study. The samples were stored in resalable vials at 5 °C in the dark but were allowed to adjust to room temperature before investigation. The testing of the antibacterial activity of different natural linden honey was carried out in vitro by the Kirby-Bauer disc diffusion technique. This study demonstrated that all samples of natural linden honey produced by Polish manufacturers demonstrated mild antibacterial activity against Staphylococcus aureus subsp. aureus Rosenbach ATCC®25923™, Pseudomonas aeruginosa (Schroeter) Migula ATCC®27853™, and Escherichia coli (Migula) Castellani and Chalmers ATCC®25922™ strains. More sensitive to all samples of natural linden honey studied was Staphylococcus aureus subsp. aureus Rosenbach ATCC®25923™ strain following Escherichia coli (Migula) Castellani and Chalmers ATCC®25922™ strain. Pseudomonas aeruginosa.