Biomarkers of Oxidative Stress in the Equine Erythrocytes after In Vitro Treatment with Extracts Derived from Different Pseudobulbs of Dendrobium parishii RCHB.F. (Orchidaceae) Plants

Abstract

The current study was conducted to investigate the antioxidant properties of extracts derived from different pseudobulbs of Dendrobium parishii Rchb.f. using biomarkers of oxidative stress (2-thiobarbituric acid reactive substances as biomarkers of lipid peroxidation, aldehydic and ketonic derivatives of oxidative modification of proteins (OMP), total antioxidant capacity (TAC)) in the equine erythrocytes after in vitro treatment with the extracts. The current study is a continuation of our cooperation with M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine (Kyiv, Ukraine) concerning investigations of antibacterial and antioxidant properties of extracts derived from leaves and pseudobulbs of some species belonging to the Orchidaceae family. The antioxidant properties of extracts derived from different pseudobulbs of D. parishii using biomarkers of oxidative stress in the equine erythrocytes after in vitro treatment with the extracts revealed that extracts derived from different pseudobulbs of D. parishii exhibited varying activity. Extracts derived from the first, second, and third pseudobulbs of D. parishii increased lipid peroxidation after in vitro treatment of equine erythrocytes. Extracts derived from the first, sixth, and seventh pseudobulbs of D. parishii caused to decrease in the levels of aldehydic derivatives of OMP. On the other hand, ketonic derivatives of OMP in the erythrocyte samples after incubation with extracts derived from all parts of pseudobulbs of D. parishii (beginning from the base of the growing tip of the rhizome) were decreased. Moreover, extracts derived from the first and sixth parts of pseudobulbs of D. parishii after incubation with erythrocyte samples caused to increase in the TAC levels. The study of extracts derived from D. parishii supports its favorable biological activities and lays a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilization of extracts of D. parishii.