Evaluation of Antibacterial Activity of the Ethanolic Extracts Derived from Leaves of Coelogyne brachyptera Rchb. f. (Orchidaceae)

Abstract

The present study was aimed to assess the antibacterial activity of ethanolic extract obtained from leaves of Coelogyne brachyptera Rchb. f. For the current study, а panel of organisms including Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 25923™) (mecA negative), S. aureus subsp. aureus Rosenbach (ATCC® 29213™) (mecA negative, Oxacillin sensitive, weak β-lactamase producing strain), S. aureus NCTC 12493 (mecA positive, Methicillin-resistant, EUCAST QC strain for cefoxitin), Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™), E. coli (Migula) Castellani and Chalmers (ATCC® 35218™), Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 27583™) were used. The antimicrobial susceptibility testing was done on Muller-Hinton agar by the disc diffusion method. The results of our study revealed the differential efficacy of ethanolic extract obtained from leaves of C. brachyptera on the test organisms. The ethanolic extract obtained from leaves of C. brachyptera revealed significant antibacterial activity against studied strains compared to control samples (96 % ethanol). A statistically significant increase (p <0.05) in the inhibition zone diameters of strain growth was 47 % for S. aureus subsp. aureus ATCC® 25923™, 40 % for S. aureus subsp. aureus ATCC® 29213™, and 44 % for S. aureus NCTC 12493. A non-significantly increase in inhibition zone diameters of E. coli strains’ growth was also observed. The plant can be a source material to the herbal drug industry since it has some important antimicrobial components in the extracts that can be used for the development of therapeutic phytomedicine and phytoveterinary.