The relevance of creating hemp hybrids using inbred lines and detecting a heterotic effect in them was caused by a need for an essentially new initial material of the crop, as well as for expanding its genetic basis and accelerating the breeding process of creating varieties through obtaining homogeneous and stable populations as early as in the initial stages. The comparative characteristics of line-variety and intervarietal hemp hybrids by productivity are presented in this article. Hybrids F1 І6 Hlukhivski 58 / Hlukhivski 51, І4 Hlesіia / Hlukhivski 51, І6 Zolotonіskі 15 / Hlukhivski 51, Hlukhivski 58 / Hlukhivski 51, Hlesіia / Hlukhivski 51, Zolotonіskі 15 / Hlukhivski 51 were studied by us. There are differences between the hybrids on the signs of the total plant height (min = 216.0, max = 298.7 cm), the technical stem length (min = 181.9, max = 220.7 cm), stem diameter (min = 8.66, max = 15.36 mm), the weight of the stem (min = 12.84, max = 50.54 g) and fiber (min = 3.59, max = 15.48 g), fiber content (min = 24.82, max = 33.00 %), the weight of seeds from one plant (min = 4.08, max = 26.72 g) and the weight of a thousand seeds (min = 17.9, max = 21.6 g). Hybrid І6 Zolotonіskі 15 / Hlukhivski 51 and a group of line-variety hybrids were the most productive. There can be recommended for practical breeding crossing of inbred lines and varieties of the Southern and middle-Russian ecology-geographical type.